Resistance testing: phenotype

Resistance testing: phenotype is a topic covered in the Johns Hopkins HIV Guide.

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DEFINITION

  • Phenotypic resistance assays use a patient’s HIV RNA plasma-derived gene sequences (RT, PR, integrase and envelope) to measure HIV replication at different concentrations of antiretroviral drugs.[3]
    • Interpretation of phenotype is based on two important clinical cutoffs: (1) lower cutoff or the value above which clinical response is diminished (partial susceptibility) and (2) upper cutoff or value above which no clinical response is expected.[2]
    • Phenotypic testing compares the activity of a patient’s HIV to a control strain with known susceptibility to a specific drug.
  • Phenotypic co-receptor tropism assays detect CCR5, CXCR4, or dual/mixed (D/M)-tropic virus. Viral tropism is the ability of HIV to bind to different co-receptors on target cells to achieve viral entry. Phenotypic co-receptor tropism assay is used to assess activity of CCR5 antagonist (maraviroc).

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DEFINITION

  • Phenotypic resistance assays use a patient’s HIV RNA plasma-derived gene sequences (RT, PR, integrase and envelope) to measure HIV replication at different concentrations of antiretroviral drugs.[3]
    • Interpretation of phenotype is based on two important clinical cutoffs: (1) lower cutoff or the value above which clinical response is diminished (partial susceptibility) and (2) upper cutoff or value above which no clinical response is expected.[2]
    • Phenotypic testing compares the activity of a patient’s HIV to a control strain with known susceptibility to a specific drug.
  • Phenotypic co-receptor tropism assays detect CCR5, CXCR4, or dual/mixed (D/M)-tropic virus. Viral tropism is the ability of HIV to bind to different co-receptors on target cells to achieve viral entry. Phenotypic co-receptor tropism assay is used to assess activity of CCR5 antagonist (maraviroc).

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Last updated: November 7, 2021