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- Aerobic, fastidious organism; easier to cultivate than M. genitalium.
- Differs from bacteria due to smaller size, presence of sterols in membrane and lack of a cell wall. The latter characteristic makes them intrinsically resistant to β-lactams.
- Differs from M. genitalium in that it is intrinsically resistant to 14-member macrolides (erythromycin and clarithromycin; see discussion below).
- M. hominis grows on agar plates and broth cultures.
- Visible colonies in 2 - 5 days.
- Colonies have "fried egg" appearance on agar (more dense in the center and less dense in the periphery).
- Colonizes male and female genitourinary tract.