Johns Hopkins ABX Guide
Acanthamoeba
Acanthamoeba is a topic covered in the Johns Hopkins ABX Guide.
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MICROBIOLOGY
- Free-living amoeba known to infect humans.[4]
- Ubiquitous; isolated worldwide from soil, air, fresh & salt water.
- Eukaryotic, shares homologies with mammalian cells.[5]
- 2 stage life cycle:
- trophozoite, actively feeding & dividing and
- dormant cyst, double-walled and wrinkled cyst, is resistant to chlorine and antibiotics. Encystation occurs under stress.
- Described as “Trojan horses,” they can harbor intracellular bacteria, known as endosymbiosis.[9]
- Laboratory culture: mumber of options available, discuss with microbiology lab.
- Non Nutrient Agar (NNA) and lawn of E. coli.
- PYG (Peptone, Yeast Extract and Glucose)
- Axenic culture, incubated on a rotary shaker with an acentric rotation of 3 cm (120 rpm/
min) at 28 C. Other variations also.
- Axenic culture, incubated on a rotary shaker with an acentric rotation of 3 cm (120 rpm/
- Jensen media
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Last updated: October 25, 2016
Citation
Spacek, Lisa A. "Acanthamoeba." Johns Hopkins ABX Guide, The Johns Hopkins University, 2016. Johns Hopkins Guide, www.hopkinsguides.com/hopkins/view/Johns_Hopkins_ABX_Guide/540002/all/Acanthamoeba.
Spacek LA. Acanthamoeba. Johns Hopkins ABX Guide. The Johns Hopkins University; 2016. https://www.hopkinsguides.com/hopkins/view/Johns_Hopkins_ABX_Guide/540002/all/Acanthamoeba. Accessed November 22, 2019.
Spacek, L. A. (2016). Acanthamoeba. In Johns Hopkins ABX Guide. Available from https://www.hopkinsguides.com/hopkins/view/Johns_Hopkins_ABX_Guide/540002/all/Acanthamoeba
Spacek LA. Acanthamoeba [Internet]. In: Johns Hopkins ABX Guide. The Johns Hopkins University; 2016. [cited 2019 November 22]. Available from: https://www.hopkinsguides.com/hopkins/view/Johns_Hopkins_ABX_Guide/540002/all/Acanthamoeba.
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TY - ELEC
T1 - Acanthamoeba
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A1 - Spacek,Lisa,M.D., Ph.D.
Y1 - 2016/10/25/
BT - Johns Hopkins ABX Guide
UR - https://www.hopkinsguides.com/hopkins/view/Johns_Hopkins_ABX_Guide/540002/all/Acanthamoeba
PB - The Johns Hopkins University
DB - Johns Hopkins Guide
DP - Unbound Medicine
ER -