Acanthamoeba is a topic covered in the Johns Hopkins ABX Guide.

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  • Free-living amoeba known to infect humans.[4]
    • Ubiquitous; isolated worldwide from soil, air, fresh & salt water.
  • Eukaryotic, shares homologies with mammalian cells.[5]
  • 2 stage life cycle:
    1. trophozoite, actively feeding & dividing and
    2. dormant cyst, double-walled and wrinkled cyst, is resistant to chlorine and antibiotics. Encystation occurs under stress.
  • Described as “Trojan horses,” they can harbor intracellular bacteria, known as endosymbiosis.[9]
  • Laboratory culture: mumber of options available, discuss with microbiology lab.
    • Non Nutrient Agar (NNA) and lawn of E. coli.
    • PYG (Peptone, Yeast Extract and Glucose)
      • Axenic culture, incubated on a rotary shaker with an acentric rotation of 3 cm (120 rpm/
        min) at 28 C. Other variations also.
    • Jensen media

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Last updated: October 25, 2016