Panton-Valentine leukocidin (PVL)-positive methicillin-susceptible and resistant Staphylococcus aureus in Taiwan: identification of oxacillin-susceptible mecA-positive methicillin-resistant S. aureus.

Authors

Chen FJ, Hiramatsu K, Huang IW, et al. 

Institution

Division of Infectious Diseases, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan, ROC.

Source

Diagn Microbiol Infect Dis 2009 Dec; 65(4) :351-7.

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) arises when methicillin-susceptible S. aureus (MSSA) acquires the staphylococcal cassette chromosome mec (SCCmec). Most pvl-positive MRSA in Taiwan belong to ST59 lineage and carry SCCmec V. The genetic profiles of 51 MSSA were compared with those of 80 MRSA from the same hospitals. Nine pvl-positive MSSA (oxacillin MIC < or = 2 microg/mL) shared >80% similarity in pulsed field gel electrophoresis pattern with 17 pvl-positive SCCmec V MRSA. Further investigation found that 5 of these 9 isolates were MRSA by cefoxitin and carried SCCmec V. All 26 pvl-positive isolates had very similar genetic profile (ST59, protein A clonal complex [spa-CC] c2:441/437, and agr group I). The success of the ST59:SCCmec V MRSA may be due in part to its heterogeneous and borderline resistance to methicillin, which may be missed by testing only oxacillin, with subsequent exposure to beta-lactams causing the emergence of more resistant subpopulations.

Mesh

Anti-Bacterial Agents
Bacterial Proteins
Bacterial Toxins
Bacterial Typing Techniques
Cefoxitin
Cluster Analysis
DNA Fingerprinting
DNA, Bacterial
Electrophoresis, Gel, Pulsed-Field
Exotoxins
Genes, Bacterial
Genotype
Hospitals
Humans
Leukocidins
Methicillin-Resistant Staphylococcus aureus
Microbial Sensitivity Tests
Oxacillin
Staphylococcal Infections
Staphylococcus aureus
Taiwan

Language

eng

Pub Type(s)

Journal Article Research Support, Non-U.S. Gov't

PubMed ID

19766426