MEDLINE Journals

    Expression of lymphocyte-endothelial receptor-ligand pairs, alpha4beta7/MAdCAM-1 and OX40/OX40 ligand in the colon and jejunum of patients with inflammatory bowel disease.

    Authors

    Souza HS, Elia CC, Spencer J, et al. 

    Source

    Gut 1999 Dec; 45(6) :856-63.

    Abstract

    BACKGROUND
    The interaction between leucocytes and vascular endothelial cells is essential for leucocyte migration into inflammatory sites.
    AIMS
    To study the local expression of the pairs of complementary molecules, alpha4beta7/mucosal addressin cell adhesion molecule (MAdCAM-1) and OX40/OX40 ligand in the lamina propria of the colon and jejunum of patients with inflammatory bowel disease.
    METHODS
    Ten patients with active ulcerative colitis (UC), nine with active Crohn's disease (CD), and seven irritable bowel syndrome (IBS) controls were submitted to endoscopic and peroral jejunal biopsies. Specimens were immunostained by indirect alkaline phosphatase using antibodies against CD3, intercellular adhesion molecule (ICAM) 1, alpha4beta7, MAdCAM-1, and OX40. An OX40-mouse-IgG fusion protein was used to detect OX40 ligand on frozen sections. Immunohistological analysis was carried out by optical microscopy using a computer assisted image analyser.
    RESULTS
    Colonic lamina propria of patients with CD and UC showed increased density of CD3+, alpha4beta7+, and OX40+ cells compared with IBS controls. ICAM-1, MAdCAM-1, and OX40 ligand positive vessels were also increased compared with IBS controls. No significant difference was found in the density of any of these cells in the jejunal mucosa of patients compared with IBS controls.
    CONCLUSIONS
    The expression of MAdCAM-1 and OX40 ligand on gut endothelial and OX40+ cells is increased in sites of mucosal inflammation in patients with inflammatory bowel disease. No evidence was found for increased lamina propria T cells or increased vascular adhesion molecule expression in the proximal intestine of patients with distal inflammatory bowel disease.

    Mesh

    Adult
    Antigens, CD27
    Antigens, CD3
    Cell Adhesion Molecules
    Colitis, Ulcerative
    Colon
    Crohn Disease
    Female
    Humans
    Immunoenzyme Techniques
    Immunoglobulins
    Inflammatory Bowel Diseases
    Integrins
    Intestinal Mucosa
    Jejunum
    Ligands
    Male
    Membrane Glycoproteins
    Middle Aged
    Mucoproteins
    OX40 Ligand
    Receptors, Lymphocyte Homing
    Receptors, OX40
    Receptors, Tumor Necrosis Factor
    T-Lymphocyte Subsets

    Language

    eng

    Pub Type(s)

    Journal Article Research Support, Non-U.S. Gov't

    PubMed ID

    10562584

    Content Manager
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